RNA polymerase
enzyme RNA polymerase or RNAP is a nucleotidyltransferase that polymerises ribonucleotides in accordance with the information present in DNA. RNA polymerase enzymes are essential and are found in all cells of all organisms.
RNAP accomplishes de novo synthesis. It is able to do this because specific interactions with the initiating nucleotide hold RNAP rigidly in place, facilitating chemical attack on the incoming nucleotide. Such specific interactions explain why RNAP prefers to start transcripts with ATP (followed by GTP, UTP, and then CTP).
RNAP was discovered independently by Sam Weiss and Jerard Hurwitz in 1960.
RNA polymerase in prokaryotes
In prokaryotes, the same enzyme catalyzes the sythesis of all three types of RNA: mRNA, rRNA and tRNA.
RNAP in E. coli is a relatively huge molecule of about 449 kD. It's core is made of 4 subunits: two α subunits, one β subunit, and one β' subunit. The RNAP holoenzyme consists of α2ββ'σ. The role of the σ subunit is to bind to a specific site of the DNA matrix, called promoter, to start the transcription. The σ subunit is released once the promoter is found. The rest of the enzyme, i.e. α2ββ', is where polymerization takes place. The structure RNAP exhibits a groove with a length of 55 Å and a diameter of 25 Å. This groove fits well the 20 Å double strand of DNA. The 55 Å length can accept 16 nucleotides.
RNA polymerase in eukaryotes
Eukaryotes have several types of RNAP:
- RNA polymerase I synthesizes precursors of rRNA.
- RNA polymerase II synthesizes precursors of mRNA. This is the most studied type, and transcription factors are required for its binding to its promoters.
- RNA polymerase III synthesizes tRNA, rRNA 5S and other small RNA's found in the nucleus and cytosol.
- Other RNA polymerase types in mitochondria and chloroplasts.
Isolation
RNA polymerase can be isolated in the following ways:
- By a phosphocellulose column (PC).
- By glycerol gradient centrifugation (GG).
- By a DNA column (A).
- PC+A
