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In the fictional Star Trek universe, the Q are a race of near-omnipotent, near-omniscient god-like beings from a parallel existence called the Q Continuum. They are largely indifferent to the affairs of the non-Q beings living in normal space, with a few exceptions. The most notable of these is Q as played by John de Lancie, a mischievous Q who periodically harasses the crews of the starships Enterprise-D and Voyager.
Other members of the Continuum featured on the series are Q's wife Q, their son q (called Junior by his father, and signified by a lower-case q in writing), Q2 who is a friend of Q, and Amanda Rogers who was raised like a human but eventually discovered her true identity. Fans have speculated that Trelane from the original series episode "The Squire of Gothos" was also a Q (see below).
Some episodes have suggested that the Q had evolved to their current state over time, that possibly they were much like humans. Q once suggested that eventually the humans might even advance beyond the Q.
Q is one of the most beloved recurring characters on Star Trek: The Next Generation, in large part due to the comedic and dramatic chemistry between actors John de Lancie and Patrick Stewart (who plays Picard, captain of the Enterprise).
†Note: Many fans have speculated that Squire Trelane was a Q; this formed the basis for Peter David's book Q-Squared. However, this link is only speculation, not canon.